8F00
Lysozyme Anomalous Dataset at 293 K and 12 keV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2020-01-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.794, 77.794, 37.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.529 - 1.200 |
| Rwork | 0.121 |
| R-free | 0.14620 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.801 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.060 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.092 | 5.750 |
| Rpim | 0.013 | 0.790 |
| Number of reflections | 34193 | 1567 |
| <I/σ(I)> | 27.9 | 1.6 |
| Completeness [%] | 95.0 | 90.1 |
| Redundancy | 53.9 | 52.8 |
| CC(1/2) | 1.000 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 5 uL of 0.6 M Sodium Chloride in 100 mM Sodium Acetate buffer pH 4.5 and 25% ethylene glycol were mixed with 5 uL 100 mg/mL lysozyme in 100 mM Sodium Acetate |
