8EZP
Lysozyme Anomalous Dataset at 260 K and 7.1 keV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 260 |
Detector technology | PIXEL |
Collection date | 2020-01-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.7462 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.502, 77.502, 37.177 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.781 - 1.900 |
Rwork | 0.130 |
R-free | 0.17250 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.570 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.781 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.048 | 0.089 |
Rpim | 0.019 | 0.030 |
Number of reflections | 9386 | 576 |
<I/σ(I)> | 40.2 | 19.7 |
Completeness [%] | 99.9 | 87.9 |
Redundancy | 11.9 | 9.7 |
CC(1/2) | 0.998 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 5 uL of 0.6 M Sodium Chloride in 100 mM Sodium Acetate buffer pH 4.5 and 25% ethylene glycol were mixed with 5 uL 100 mg/mL lysozyme in 100 mM Sodium Acetate. |