8EZO

Lysozyme Anomalous Dataset at 220 K and 7.1 keV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	220
Detector technology	PIXEL
Collection date	2020-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.7462
Spacegroup name	P 43 21 2
Unit cell lengths	76.909, 76.909, 36.961
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.484 - 1.900
Rwork	0.156
R-free	0.19130
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.633
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.484	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.251
Rpim	0.015	0.102
Number of reflections	9211	580
<I/σ(I)>	37.2	8.5
Completeness [%]	99.9	98.2
Redundancy	15.6	11.6
CC(1/2)	0.999	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	5 uL of 0.6 M Sodium Chloride in 100 mM Sodium Acetate buffer pH 4.5 and 25% ethylene glycol were mixed with 5 uL 100 mg/mL lysozyme in 100 mM Sodium Acetate.