8EZM

Pfs25 in complex with transmission-reducing antibody AS01-63

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	101.890, 84.710, 65.870
Unit cell angles	90.00, 96.57, 90.00

Refinement procedure

Resolution	44.500 - 2.100
R-factor	0.2066
Rwork	0.204
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6phb
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	2.175
High resolution limit [Å]	2.100	2.100
Rpim	0.027	0.357
Number of reflections	30366	2543
<I/σ(I)>	14
Completeness [%]	93.2	77.68
Redundancy	3.5	3.5
CC(1/2)	0.998	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	291	2.2M ammonium sulfate, 0.1M sodium citrate pH 5.4