8EY8

LaM domain of human LARP1 in complex with AAAAGA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-27
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95371
Spacegroup name	P 21 21 21
Unit cell lengths	36.683, 45.879, 58.861
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.190 - 1.300
R-factor	0.1835
Rwork	0.183
R-free	0.19210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.014
RMSD bond angle	1.589
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.320
High resolution limit [Å]	1.300	1.300
Number of reflections	23947	794
<I/σ(I)>	22.1	2.2
Completeness [%]	95.5	65.5
Redundancy	6.4	2.8
CC(1/2)	0.996	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.2 M ammonium acetate, 0.1 M Bis-Tris pH 6.5, 25% (w/v) PEG 3350