8EY7

LaM domain of human LARP1 in complex with AAAGAA RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95371
Spacegroup name	P 21 21 21
Unit cell lengths	36.908, 45.338, 59.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.620 - 1.350
R-factor	0.1879
Rwork	0.186
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7soo
RMSD bond length	0.013
RMSD bond angle	1.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	20906	636
<I/σ(I)>	17.7	1.7
Completeness [%]	93.3	57.6
Redundancy	7.1	5
CC(1/2)	0.998	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.056 M sodium phosphate, 1.344 M potassium phosphate