8EY4
Contact-dependent growth inhibition toxin-immunity protein complex from E. coli O32:H37
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.641, 53.660, 38.533 |
| Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
| Resolution | 28.710 - 1.830 |
| R-factor | 0.161 |
| Rwork | 0.157 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2947) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.078 | 0.358 |
| Number of reflections | 12331 | 565 |
| <I/σ(I)> | 25 | 2.5 |
| Completeness [%] | 99.3 | 92 |
| Redundancy | 4.8 | 3.1 |
| CC(1/2) | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25.7% PEG 5K MME, 150 mM MES pH 6.0, 6% propanediol, cryo 10% glycerol |
