8EXE

Crystal structure of human FAM46A-BCCIPa complex at 3.5 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	87.800, 88.152, 102.981
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.460 - 3.500
R-factor	0.2624
Rwork	0.260
R-free	0.29740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6w36
RMSD bond length	0.004
RMSD bond angle	0.681
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.165	1.277
Rpim	0.088	0.670
Number of reflections	10371	478
<I/σ(I)>	7.9
Completeness [%]	96.3	90.2
Redundancy	4.1
CC(1/2)	0.981	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M Tris pH 7.5, 0.4 % v/v Jeffamine ED-2001, and 0.5-0.6 M Sodium malonate