8EXD
Crystal structure of Aspergillus fumigatus sterylglucosidase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2021-11-06 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0331 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.731, 112.792, 139.102 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 58.210 - 3.800 |
| R-factor | 0.2807 |
| Rwork | 0.279 |
| R-free | 0.32360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lpq |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 76.950 | 76.950 | 4.010 |
| High resolution limit [Å] | 3.800 | 12.020 | 3.800 |
| Rmerge | 0.123 | 0.066 | 0.840 |
| Number of reflections | 32036 | 1064 | 4666 |
| <I/σ(I)> | 7.7 | ||
| Completeness [%] | 99.3 | 99.5 | 99.8 |
| Redundancy | 7 | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 20% polyethylene glycol (PEG) 3350, 0.2 M magnesium acetate pH 4.5 |
