8EWW

Structure of Arabidopsis fatty acid amide hydrolase mutant S305A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	93
Detector technology	CCD
Collection date	2019-07-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.078090
Spacegroup name	P 1 21 1
Unit cell lengths	72.451, 81.324, 133.180
Unit cell angles	90.00, 104.51, 90.00

Refinement procedure

Resolution	38.780 - 2.800
R-factor	0.2355
Rwork	0.234
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dhv
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.800	2.920
High resolution limit [Å]	2.800	2.800
Rmerge	0.044	0.590
Number of reflections	36725	4438
<I/σ(I)>	9.1	1.4
Completeness [%]	98.8	98.1
Redundancy	3.5	3.5
CC(1/2)	0.960	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1 M MES, PH 6.0, 30% PEG 200, 5% PEG 3350