8EWO

Crystal structure of putative glyoxylase II from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-09-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	70.723, 76.608, 75.430
Unit cell angles	90.00, 94.63, 90.00

Refinement procedure

Resolution	28.710 - 2.470
R-factor	0.1812
Rwork	0.179
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xm8
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.510
High resolution limit [Å]	2.470	2.470
Rmerge	0.121	0.363
Rpim	0.068	0.263
Number of reflections	28275	1138
<I/σ(I)>	7.68	1.28
Completeness [%]	98.2	80.4
Redundancy	3.8	2.2
CC(1/2)	0.825	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M magnesium acetate, 25% PEG3350, cryoprotectant: 6% glycerol