8EVM
De novo design of chlorophyll special pair containing protein assemblies
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.732, 88.732, 149.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.370 - 3.050 |
| R-factor | 0.253 |
| Rwork | 0.248 |
| R-free | 0.29730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Designed Protein |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.517 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc2_3793) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.370 | 3.150 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.194 | |
| Number of reflections | 13483 | 1311 |
| <I/σ(I)> | 11.54 | 0.62 |
| Completeness [%] | 99.4 | 96.27 |
| Redundancy | 10.8 | 10.7 |
| CC(1/2) | 0.999 | 0.248 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.4 M Sodium malonate dibasic monohydrate pH 7.0 |
