8EVL
Crystal structure of alpha-COPI N-terminal WD40 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.687, 171.416, 69.991 |
| Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
| Resolution | 29.660 - 1.900 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7s22 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.662 |
| Data reduction software | HKL-3000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.660 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 67199 | 6741 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 3.7 |
| CC(1/2) | 0.993 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.2M Sodium citrate tribasic dihydrate, 18% PEG3350 |
