8EVL

Crystal structure of alpha-COPI N-terminal WD40 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	P 1 21 1
Unit cell lengths	36.687, 171.416, 69.991
Unit cell angles	90.00, 97.34, 90.00

Refinement procedure

Resolution	29.660 - 1.900
R-factor	0.1938
Rwork	0.192
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7s22
RMSD bond length	0.003
RMSD bond angle	0.662
Data reduction software	HKL-3000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.660	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	67199	6741
<I/σ(I)>	5.7
Completeness [%]	100.0	100
Redundancy	4	3.7
CC(1/2)	0.993	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	0.2M Sodium citrate tribasic dihydrate, 18% PEG3350