8ESE
Crystal structure of human Vps29 bound to a peptide from Vps35L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.95365 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.719, 55.285, 82.644 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.950 - 1.350 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xsa |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.944 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20rc3_4406) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.950 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rpim | 0.031 | 0.643 |
| Number of reflections | 44459 | 1992 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.998 | 0.481 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Bis-tris pH 5.5 and 25% (w/v) PEG3350 |
