8ES5

Aha1 domain protein from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-21
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.95369
Spacegroup name	I 2 2 2
Unit cell lengths	55.250, 80.770, 161.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.380 - 1.970
R-factor	0.1952
Rwork	0.193
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q6a
RMSD bond length	0.020
RMSD bond angle	1.577
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.380	2.041
High resolution limit [Å]	1.970	1.970
Rmerge	0.018
Rpim	0.018
Number of reflections	39557	2215
<I/σ(I)>	17.15
Completeness [%]	100.0	86.28
Redundancy	2
CC(1/2)	1.000	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.1 M Sodium malonate, 0.1 M HEPES:NaOH, 0.5% (w/v) Jeffamine ED-2003