8ERI

HTLV-1 capsid protein full-length

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-15
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	F 2 2 2
Unit cell lengths	74.079, 128.663, 297.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.630 - 2.250
R-factor	0.2528
Rwork	0.251
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2 prediction
Data reduction software	XDS
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.640	48.640	2.320
High resolution limit [Å]	2.250	9.000	2.250
Rmerge	0.142	0.079	1.222
Rmeas	0.148	0.083	1.271
Rpim	0.040	0.022	0.346
Total number of observations	454329	7379	36962
Number of reflections	33745	593	2877
<I/σ(I)>	8.5	25.2	1.2
Completeness [%]	99.4	99.1	93.2
Redundancy	13.5	12.4	12.8
CC(1/2)	0.998	0.997	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M Na citrate, pH 6.0 8% 2-propanol 23% PEG3350