8ERB

Crystal structure of Fub7 in complex with vinylglycine ketimine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-01-28
Detector	RAYONIX MX-325
Wavelength(s)	1.1950
Spacegroup name	P 1 21 1
Unit cell lengths	146.703, 193.364, 149.913
Unit cell angles	90.00, 99.60, 90.00

Refinement procedure

Resolution	47.750 - 1.980
R-factor	0.2017
Rwork	0.200
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8eqw
RMSD bond length	0.010
RMSD bond angle	1.255
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.010
High resolution limit [Å]	1.980	1.980
Number of reflections	565830	28182
<I/σ(I)>	16.1
Completeness [%]	99.8
Redundancy	7.1
CC(1/2)	0.965	0.755

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M sodium malonate, pH 6.0, 25% PEG 3350, crystals soaked with 2 mM O-acetyl-L-homoserine for 5 min.