8ER5
Crystal Structure of NlpC/P60 domain from Clostridium innocuum NlpC/P60 domain-containing protein CI_01448.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-11-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 51.954, 51.954, 157.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.620 - 1.920 |
R-factor | 0.1584 |
Rwork | 0.156 |
R-free | 0.20300 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.311 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.137 | 0.856 |
Rmeas | 0.149 | 0.928 |
Rpim | 0.058 | 0.357 |
Number of reflections | 19632 | 959 |
<I/σ(I)> | 15.2 | 2.6 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 6.5 | 6.5 |
CC(1/2) | 0.988 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | Protein: 10.0 mg/ml, 0.25M Sodium chloride, 0.01M Tris pH 8.3; Screen: AmSO4 (G4), 1.0M Ammonium sulfate, 0.1M HEPES sodium salt pH 5.6, 2% (w/v) PEG 400; Cryo: 2M Lithium sulfate |