8EQH
The crystal structure of 14-3-3 Beta containing 3-nitrotyrosine at position Y213
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 96 |
Detector technology | PIXEL |
Collection date | 2019-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 85.290, 115.030, 79.490 |
Unit cell angles | 90.00, 119.54, 90.00 |
Refinement procedure
Resolution | 44.220 - 1.900 |
R-factor | 0.1891 |
Rwork | 0.188 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bq0 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.721 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.220 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 52427 | 3903 |
<I/σ(I)> | 16.48 | |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 26.3 | |
CC(1/2) | 1.000 | 0.197 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20%-27% PEG 3350, +/- 50 mM MgCl2, 100 mM Bis-Tris pH 5.5-6.5 |