8EPB

Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-03-27
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 32
Unit cell lengths	37.970, 37.970, 69.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.730 - 2.610
R-factor	0.2197
Rwork	0.216
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ideal B type DNA duplexes generated in Cadnano
RMSD bond length	0.013
RMSD bond angle	1.715
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.883	2.948
High resolution limit [Å]	2.610	2.610
Number of reflections	1876	208
<I/σ(I)>	17.3
Completeness [%]	80.0
Redundancy	7.5
CC(1/2)	1.000	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.5 ug/uL motif 0.005 M magnesium sulfate hydrate, 0.005 M HEPES sodium, 0.16 M lithium sulfate monohydrate, 3 % MPD at pH 7.0