8EP8
Engineering Crystals with Tunable Symmetries from 14- or 16-Base-Long DNA Strands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2022-05-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 32.998, 32.998, 104.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 2.450 |
| R-factor | 0.2507 |
| Rwork | 0.250 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.965 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 3.062 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 1206 | 202 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 85.7 | |
| Redundancy | 11.4 | 9.1 |
| CC(1/2) | 1.000 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.5 ug/uL motif 0.005 M magnesium sulfate hydrate, 0.005 M HEPES sodium pH 7.0, 0.16 M lithium sulfate monohydrate, 4 % MPD at pH 7.0 |
