8EOX

Precisely patterned nanofibers made from extendable protein multiplexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	62.755, 80.364, 66.705
Unit cell angles	90.00, 112.75, 90.00

Refinement procedure

Resolution	53.790 - 3.300
R-factor	0.2973
Rwork	0.295
R-free	0.35190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Designed Model
RMSD bond length	0.002
RMSD bond angle	0.451
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.790	3.400
High resolution limit [Å]	3.300	3.300
Rmerge	0.035	0.700
Number of reflections	9068	907
<I/σ(I)>	7.22	0.9
Completeness [%]	97.0	98.69
Redundancy	1.9	2
CC(1/2)	0.997	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02M of carboxylic acids, & 0.1M MOPS/HEPES-Na pH7.5