8ENY
Crystal structure of alpha-COPI-WD40 domain R13A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.444, 170.204, 71.524 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 28.370 - 1.900 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7s22 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.645 |
| Data reduction software | HKL-3000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 67929 | 4533 |
| <I/σ(I)> | 5.9 | 1.4 |
| Completeness [%] | 98.2 | 97.1 |
| Redundancy | 4.8 | 4.4 |
| CC(1/2) | 0.994 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.14M Sodium citrate tribasic dihydrate, 16% PEG3350 |
