8ENH

Cross-reactive 3180 TCR recognition of HLA-B*35:01-NP7 epitope from 2002 H3N2 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-01-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95365
Spacegroup name	P 1 21 1
Unit cell lengths	168.150, 79.550, 172.190
Unit cell angles	90.00, 95.56, 90.00

Refinement procedure

Resolution	49.465 - 2.500
R-factor	0.192799955749
Rwork	0.191
R-free	0.23030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.013
RMSD bond angle	1.586
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.590
High resolution limit [Å]	2.500	2.500
Rmeas	0.053	0.406
Number of reflections	156773	15158
<I/σ(I)>	13.5	2.7
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.998	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG, ACETATE