8ENB

Crystal structure of LGR ligand alpha2/beta5 from C. elegans in crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2020-10-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	52.371, 90.748, 59.563
Unit cell angles	90.00, 97.18, 90.00

Refinement procedure

Resolution	49.520 - 2.350
R-factor	0.2407
Rwork	0.238
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	STARANISO
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	2.470
High resolution limit [Å]	2.300	2.300
Number of reflections	15726	787
<I/σ(I)>	6.4
Completeness [%]	86.2
Redundancy	4.9
CC(1/2)	0.990	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 12% w/v PEG6000