8EMY

Structure of GII.4 norovirus in complex with Nanobody 82

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	121.021, 142.459, 207.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.390 - 1.700
R-factor	0.1773
Rwork	0.177
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oos
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	117.530	117.530	1.730
High resolution limit [Å]	1.700	9.300	1.700
Rmerge	0.040	0.018	0.388
Rmeas	0.046	0.021	0.456
Rpim	0.022	0.011	0.233
Total number of observations	1649444	10277	54689
Number of reflections	380256	2485	14799
<I/σ(I)>	19.1	48.5	2.9
Completeness [%]	96.9	94.9	77
Redundancy	4.3	4.1	3.7
CC(1/2)	0.991	0.960	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	5% [w/v] PEG 6000 and 0.1M HEPES [pH 7.0]