8EML

Crystal Structure of Gsx2 Homeodomain in Complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	80
Detector technology	CCD
Collection date	2021-11-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	37.700, 37.650, 107.869
Unit cell angles	90.00, 93.99, 90.00

Refinement procedure

Resolution	36.300 - 2.210
R-factor	0.2247
Rwork	0.220
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h1k
RMSD bond length	0.013
RMSD bond angle	1.514
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.430	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	15221	1230
<I/σ(I)>	5.1
Completeness [%]	99.7	97.2
Redundancy	3.4	2.7
CC(1/2)	0.990	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1M Magnesium Chloride Hexahydrate, 0.1M Rubidium Chloride, 0.1M HEPES pH 7.5, 30% PEG Smear Broad