8EMF

Crystal structure of a HLA-B*35:01-NP6 epitope from 1977 H1N1 influenza strain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	P 21 21 21
Unit cell lengths	51.000, 81.645, 109.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.899 - 1.805
R-factor	0.180050003169
Rwork	0.179
R-free	0.20317
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lks
RMSD bond length	0.011
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.900	1.870
High resolution limit [Å]	1.800	1.810
Rmeas	0.037	0.310
Number of reflections	42596	4086
<I/σ(I)>	19.6
Completeness [%]	100.0	97
Redundancy	2
CC(1/2)	0.999	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	PEG