8EMB
X-ray crystal structure of Thermosynechococcus elongatus Si3 domain of RNA polymerase RpoC2 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 178.663, 178.663, 281.038 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.610 - 3.060 |
| R-factor | 0.2648 |
| Rwork | 0.264 |
| R-free | 0.28450 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.120 |
| High resolution limit [Å] | 3.060 | 3.070 |
| Rmeas | 0.209 | |
| Rpim | 0.060 | |
| Number of reflections | 96754 | 4161 |
| <I/σ(I)> | 17 | |
| Completeness [%] | 98.1 | 85.8 |
| Redundancy | 12 | 9.5 |
| CC(1/2) | 0.996 | 0.263 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 278 | 0.1 M sodium citrate, pH 5, 0.4-0.6 M magnesium chloride, 4-6% PEG3350, 50 ug/mL heparin |
