8EM9

Human PU.1 ETS-Domain (165-270) Bound to d(AATAGGAGAAGTAGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920119
Spacegroup name	P 32
Unit cell lengths	46.184, 46.184, 90.992
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.170 - 2.341
R-factor	0.2272
Rwork	0.223
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.170	2.424
High resolution limit [Å]	2.341	2.341
Rmerge	0.097	0.843
Rmeas	0.106	0.919
Rpim	0.042	0.364
Number of reflections	9146	942
<I/σ(I)>	15.15	2.44
Completeness [%]	99.6	99.79
Redundancy	6.6	6.5
CC(1/2)	0.995	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350