8ELY

HRAS R97M Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	39.241, 39.241, 157.693
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.980 - 1.750
Rwork	0.179
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.079	0.072	0.219
Rmeas	0.084	0.077	0.260
Rpim	0.029	0.027	0.137
Total number of observations	100390
Number of reflections	13525	872	277
<I/σ(I)>	23.7
Completeness [%]	89.3	98.9	37.3
Redundancy	7.4	7.9	3.4
CC(1/2)		0.995	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT