8ELY
HRAS R97M Crystal Form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.241, 39.241, 157.693 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.980 - 1.750 |
| Rwork | 0.179 |
| R-free | 0.22680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctq |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.079 | 0.072 | 0.219 |
| Rmeas | 0.084 | 0.077 | 0.260 |
| Rpim | 0.029 | 0.027 | 0.137 |
| Total number of observations | 100390 | ||
| Number of reflections | 13525 | 872 | 277 |
| <I/σ(I)> | 23.7 | ||
| Completeness [%] | 89.3 | 98.9 | 37.3 |
| Redundancy | 7.4 | 7.9 | 3.4 |
| CC(1/2) | 0.995 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT |
