8ELV

HRAS R97I Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-02-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	38.999, 38.999, 158.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.770 - 2.153
Rwork	0.184
R-free	0.26980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.190
High resolution limit [Å]	2.150	5.830	2.150
Rmerge	0.089	0.045	0.528
Rmeas	0.095	0.048	0.658
Rpim	0.032	0.017	0.385
Total number of observations	63965
Number of reflections	8021	498	305
<I/σ(I)>	7.9
Completeness [%]	97.3	99.8	77
Redundancy	8	8.1	2.2
CC(1/2)		0.997	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT