8ELV
HRAS R97I Crystal Form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-02-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.999, 38.999, 158.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.770 - 2.153 |
| Rwork | 0.184 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctq |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
| Rmerge | 0.089 | 0.045 | 0.528 |
| Rmeas | 0.095 | 0.048 | 0.658 |
| Rpim | 0.032 | 0.017 | 0.385 |
| Total number of observations | 63965 | ||
| Number of reflections | 8021 | 498 | 305 |
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 97.3 | 99.8 | 77 |
| Redundancy | 8 | 8.1 | 2.2 |
| CC(1/2) | 0.997 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT |
