8ELT
HRAS R97G Crystal Form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-02-12 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.941, 38.941, 157.386 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.720 - 1.660 |
| Rwork | 0.198 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctq |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.690 |
| High resolution limit [Å] | 1.660 | 4.500 | 1.660 |
| Rmerge | 0.055 | 0.036 | 0.790 |
| Rmeas | 0.058 | 0.038 | 0.862 |
| Rpim | 0.019 | 0.012 | 0.338 |
| Total number of observations | 160772 | ||
| Number of reflections | 17212 | 1001 | 883 |
| <I/σ(I)> | 10 | ||
| Completeness [%] | 99.4 | 99.7 | 99.2 |
| Redundancy | 9.3 | 8.8 | 6.1 |
| CC(1/2) | 0.999 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT |
