8ELT

HRAS R97G Crystal Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 32 2 1
Unit cell lengths	38.941, 38.941, 157.386
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.720 - 1.660
Rwork	0.198
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctq
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.690
High resolution limit [Å]	1.660	4.500	1.660
Rmerge	0.055	0.036	0.790
Rmeas	0.058	0.038	0.862
Rpim	0.019	0.012	0.338
Total number of observations	160772
Number of reflections	17212	1001	883
<I/σ(I)>	10
Completeness [%]	99.4	99.7	99.2
Redundancy	9.3	8.8	6.1
CC(1/2)		0.999	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Calcium Acetate, Magnesium Chloride, Sodium Chloride, DTT