8ELF

Structure of Get3d, a homolog of Get3, from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	59.251, 67.050, 99.398
Unit cell angles	90.00, 97.81, 90.00

Refinement procedure

Resolution	32.830 - 2.000
R-factor	0.2156
Rwork	0.213
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3igf
RMSD bond length	0.003
RMSD bond angle	0.511
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.700	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.061	1.863
Rmeas	0.070
Rpim	0.034	1.037
Number of reflections	52116	16240
<I/σ(I)>	10.3
Completeness [%]	99.5
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.47	291	Protein solution: 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM DTT, 1 mM MgCl2, 10% glycerol, 2 mM ADP/AMP-PNP. Reservoir solution: 50 mM sodium cacodylate (pH 5.47), 50 mM lithium sulfate, 30% PEG-4000