8ELB

CTX-M-14 beta-lactamase mutant- N132A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00001
Spacegroup name	C 2 2 21
Unit cell lengths	59.230, 60.410, 264.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.290 - 1.500
R-factor	0.19101
Rwork	0.189
R-free	0.22212
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ylt
RMSD bond length	0.035
RMSD bond angle	2.122
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.290	1.520
High resolution limit [Å]	1.500	1.500
Rmerge	0.183	0.118
Rmeas		0.132
Rpim	0.084	0.058
Number of reflections	69726	2475
<I/σ(I)>	6	7.9
Completeness [%]	95.3	95.3
Redundancy	5.9
CC(1/2)	0.975	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	296	0.1 M Tris-HCl, PEG 10,000 (20% w/v)