8EL6

Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 with an altered helix hA/hY conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-23
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	61.708, 70.001, 48.019
Unit cell angles	90.00, 110.31, 90.00

Refinement procedure

Resolution	45.030 - 1.950
R-factor	0.1857
Rwork	0.181
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmx
RMSD bond length	0.013
RMSD bond angle	1.504
Data reduction software	DIALS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.030	1.870
High resolution limit [Å]	1.830	1.830
Rmerge	0.210	1.539
Number of reflections	33901	2072
<I/σ(I)>	6.9	1.2
Completeness [%]	100.0
Redundancy	7.3
CC(1/2)	0.994	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	PEG3350 23.6% (w/v), 150 nL mother + 150 nL protein solution, Cryoprotectant of Paratone-N