8EL5
Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 in an alternating tight to loose interface filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.856, 75.596, 99.360 |
| Unit cell angles | 90.00, 110.30, 90.00 |
Refinement procedure
| Resolution | 61.660 - 1.670 |
| R-factor | 0.1866 |
| Rwork | 0.186 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lmx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.446 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHENIX (1.14) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.700 | 1.700 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.091 | |
| Number of reflections | 104433 | 5125 |
| <I/σ(I)> | 9.5 | 0.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.406 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 25% PEG3350 (w/v) + 2% (w/v) Benzamidine HCl, 150 nL mother + 150 nl protein solution, Cryoprotectant of 20% Gylcerol |
