8EL3

Light harvesting phycobiliprotein HaPE555 from the cryptophyte Hemiselmis andersenii CCMP644 in a loose interface filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	64.802, 76.840, 103.360
Unit cell angles	90.00, 110.73, 90.00

Refinement procedure

Resolution	48.330 - 1.570
R-factor	0.1706
Rwork	0.170
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmx
RMSD bond length	0.013
RMSD bond angle	1.385
Data reduction software	DIALS
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.14)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.330	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.095	1.399
Number of reflections	129756	5326
<I/σ(I)>	10.5	1
Completeness [%]	98.2
Redundancy	6.4
CC(1/2)	0.999	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	PEG3350 25% (w/v) + NaBr 0.01M, 150nL mother + 150 nL protein stock, Cryoprotectant of 20% Glycerol