8EKZ

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGGAGAAGTAGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-05-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000030
Spacegroup name	P 1 21 1
Unit cell lengths	43.146, 60.993, 44.429
Unit cell angles	90.00, 116.58, 90.00

Refinement procedure

Resolution	23.010 - 1.420
R-factor	0.1537
Rwork	0.153
R-free	0.16590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.009
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.010	1.471
High resolution limit [Å]	1.420	1.420
Rmerge	0.035	0.297
Rmeas	0.040	0.340
Rpim	0.020	0.165
Number of reflections	36849	3684
<I/σ(I)>	22.83	4.74
Completeness [%]	94.5	94.77
Redundancy	4.1	4.2
CC(1/2)	0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350