8EKU

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAATGGAATGGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920078
Spacegroup name	P 1 21 1
Unit cell lengths	42.757, 60.707, 44.675
Unit cell angles	90.00, 116.87, 90.00

Refinement procedure

Resolution	33.310 - 1.520
R-factor	0.159
Rwork	0.157
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.010
RMSD bond angle	1.314
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.310	1.574
High resolution limit [Å]	1.520	1.520
Rmerge	0.059	0.373
Rmeas	0.070	0.444
Rpim	0.036	0.238
Number of reflections	31260	3107
<I/σ(I)>	11.81	2.94
Completeness [%]	99.3	99.81
Redundancy	3.5	3.4
CC(1/2)	0.997	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350