8EJ6
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGAGGAATGGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.920099 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.957, 61.486, 44.387 |
Unit cell angles | 90.00, 117.25, 90.00 |
Refinement procedure
Resolution | 24.250 - 1.390 |
R-factor | 0.1444 |
Rwork | 0.143 |
R-free | 0.16770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8e3k |
RMSD bond length | 0.008 |
RMSD bond angle | 1.151 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.250 | 1.440 |
High resolution limit [Å] | 1.390 | 1.390 |
Rmerge | 0.078 | 0.357 |
Rmeas | 0.090 | 0.410 |
Rpim | 0.043 | 0.200 |
Number of reflections | 41237 | 4083 |
<I/σ(I)> | 11.72 | 2.71 |
Completeness [%] | 99.7 | 99.76 |
Redundancy | 4.2 | 4.1 |
CC(1/2) | 0.995 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |