8EIC

Crystal structure of beta-catenin and the MDM2 p53-binding domain in complex with H330, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	56.110, 71.540, 164.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.530 - 2.620
R-factor	0.228
Rwork	0.226
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uwi
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	2.740
High resolution limit [Å]	2.620	2.620
Rmerge	0.139
Number of reflections	20670	2482
<I/σ(I)>	14.4	2.4
Completeness [%]	99.9
Redundancy	12.9
CC(1/2)	0.999	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4M Sodium formate