8EI0
Crystal structure of the STUB1 TPR domain in complex with H318, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.560, 46.960, 53.620 |
| Unit cell angles | 90.00, 126.48, 90.00 |
Refinement procedure
| Resolution | 26.750 - 1.470 |
| R-factor | 0.1931 |
| Rwork | 0.192 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5NSV |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.110 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.036 | 0.650 |
| Number of reflections | 27179 | 1284 |
| <I/σ(I)> | 18.3 | 2.2 |
| Completeness [%] | 96.7 | 94.5 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ammonium sulfate, 30% w/v PEG 8000 |
