8EGK
Re-refinement of Crystal Structure of NosGet3d, the All4481 protein from Nostoc sp. PCC 7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.170, 55.553, 122.363 |
| Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
| Resolution | 27.520 - 1.980 |
| R-factor | 0.1905 |
| Rwork | 0.189 |
| R-free | 0.22090 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3igf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.520 | 2.070 |
| High resolution limit [Å] | 1.980 | 2.000 |
| Rmerge | 0.060 | 0.288 |
| Number of reflections | 110202 | 11087 |
| <I/σ(I)> | 18.8 | |
| Completeness [%] | 89.2 | |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: 100mM MES (pH 6.15), 18% PEG3350, and 100mM potassium nitrate |
