8EF3

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ119-D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-16
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	70.243, 71.015, 103.741
Unit cell angles	90.00, 102.38, 90.00

Refinement procedure

Resolution	34.305 - 1.672
R-factor	0.1822
Rwork	0.182
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tz2 5qf1
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.670	1.670
Number of reflections	114507	11124
<I/σ(I)>	21.2
Completeness [%]	99.3
Redundancy	3.7
CC(1/2)	0.850	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	18% PEG 8K, 0.2M CaAc hydrate, 0.1M Na Cacodylate (pH6.5)