8EF2

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ107-B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	41.235, 118.672, 119.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.834 - 2.102
R-factor	0.1744
Rwork	0.172
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fqq
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	32733	2905
<I/σ(I)>	9.4
Completeness [%]	93.2
Redundancy	4
CC(1/2)	0.860	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	23.5% PEG 4K, 0.2 M (NH4)2SO4