8EEO

C. ammoniagenes monoamine oxidase bound to cadaverine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	64.408, 98.986, 141.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.590 - 1.940
R-factor	0.1797
Rwork	0.179
R-free	0.19770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.512
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.590	2.009
High resolution limit [Å]	1.940	1.940
Rmerge	0.142	0.946
Rmeas	0.145	0.971
Rpim	0.029	0.203
Number of reflections	67161	6137
<I/σ(I)>	18.3	3.3
Completeness [%]	99.1	91.42
Redundancy	15.3	10.7
CC(1/2)	0.999	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	10 - 15% w/v PEG-3350, 0.1 M sodium malonate, pH 5.0