8EEK

C. ammoniagenes monoamine oxidase (MAO) bound to tyramine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	59.881, 118.600, 141.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.070 - 1.510
R-factor	0.228
Rwork	0.228
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.036
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.070	1.564
High resolution limit [Å]	1.510	1.510
Rmerge	0.272	1.371
Rmeas	0.278
Rpim	0.054	0.489
Number of reflections	157374	15566
<I/σ(I)>	15	1.1
Completeness [%]	99.2	99.34
Redundancy	25.8	25.2
CC(1/2)	0.999	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	10 - 15% w/v PEG-3350, 0.1 M sodium malonate, pH 5.0