8EEE

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ104-d in complex with ZIKV E glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	85.320, 130.420, 109.710
Unit cell angles	90.00, 104.17, 90.00

Refinement procedure

Resolution	14.989 - 2.819
R-factor	0.2196
Rwork	0.217
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ire
RMSD bond length	0.003
RMSD bond angle	0.836
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	50533	1890
<I/σ(I)>	14.5
Completeness [%]	89.0
Redundancy	4.6
CC(1/2)	0.930	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12% PEG 8000, 0.2 M (NH4)2SO4, 0.1M Tris-HCl (pH 8.5)