8EDV

Mitoguardin homolog (MIGA) delta TM residues 106-496 from Caenorhabditis elegans bound to modelled lipid phosphatidylethanolamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979110
Spacegroup name	P 31 2 1
Unit cell lengths	91.278, 91.278, 366.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.350 - 3.300
R-factor	0.2711
Rwork	0.270
R-free	0.29560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 model
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.169	0.736
Rmeas	0.179	0.774
Rpim	0.056	0.236
Number of reflections	27736	2670
<I/σ(I)>	14
Completeness [%]	99.9	99.2
Redundancy	10.2	10.5
CC(1/2)	0.993	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	0.2 M sodium malonate pH 7.4, 22% PEG3350